A synthetic peptide is almost never supplied as a free base. It arrives as a salt, and which salt it is affects both your concentration calculations and, in some assay systems…
Weighing a lyophilised peptide accurately is harder than it looks, and the errors are systematic rather than random — they push in one direction and quietly bias every…
Freeze-drying removes most of the water from a peptide preparation. It does not remove all of it…
A vial labelled 10 mg does not contain 10 mg of peptide. This is not a supplier shortcut — it follows from how peptides are purified and dried — but it is routinely missed…
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