NAD+ 1000mg

Description

NAD+ 1000mg – Nicotinamide Adenine Dinucleotide for Laboratory Research

NAD⁺ is the oxidised form of nicotinamide adenine dinucleotide, a coenzyme found in every living cell. It is not a peptide. It is two nucleotides, nicotinamide riboside and adenosine monophosphate, joined through their phosphate groups by a pyrophosphate bridge. That structure lets it do two very different jobs in biochemistry, which is why it appears in so many laboratory models. Supplied at 99%+ purity.

NAD+ 1000mg is supplied as a dry powder for in vitro and laboratory research. It is not approved for human or veterinary use.

Two roles, one molecule

As a redox carrier. The nicotinamide ring accepts a hydride ion (two electrons and a proton) to become NADH, and gives it up again when NADH is oxidised. Dehydrogenases in glycolysis, the citric acid cycle and fatty-acid oxidation use the NAD⁺/NADH pair to move electrons toward the mitochondrial respiratory chain. In this role the molecule is recycled, not used up.

As a substrate that is consumed. Three enzyme families break NAD⁺ apart, splitting off nicotinamide and using the ADP-ribose part:

• Sirtuins (SIRT1–7) remove acetyl and other acyl groups from proteins, producing nicotinamide and O-acyl-ADP-ribose.
• PARPs (poly(ADP-ribose) polymerases) attach chains of ADP-ribose to proteins, notably at sites of DNA damage.
• CD38 and CD157 hydrolyse NAD⁺ and make the signalling molecule cyclic ADP-ribose.

Because these consumers compete for one pool, NAD⁺ availability is itself a variable in cell models. That is what reviews such as Covarrubias et al. (2021) focus on: how synthesis, recycling through the salvage pathway, and consumption balance out in different cell types.

Stability: the property that matters most in practice

The oxidised and reduced forms fail in opposite conditions, a finding that goes back to Lowry and colleagues’ work on pyridine nucleotide stability in 1961:

• NAD⁺ is labile in alkaline solution and comparatively stable in mild acid.
• NADH is labile in acid and comparatively stable in mild alkali.

For NAD⁺ that means preparing solutions in neutral to slightly acidic buffer, keeping them cold, and making them fresh rather than storing them for long. The dry powder is hygroscopic, so let the vial reach room temperature before opening to stop moisture condensing on it, and reseal it promptly.

A large-format vial

At 1,000 mg, this vial suits work that consumes the compound quickly, such as preparing concentrated stocks for enzyme assays or running many conditions from one batch. Using one batch across a whole series removes batch-to-batch variation as a variable. If the full quantity will not be used soon, weigh out portions under dry conditions rather than opening and reclosing the main vial repeatedly.

Questions researchers ask

Is NAD+ a peptide? No. It is a dinucleotide coenzyme, formula C₂₁H₂₇N₇O₁₄P₂, 663.4 g/mol. It is sold alongside peptides because the same laboratories use both.

How is NAD+ told apart from NADH? Both absorb ultraviolet light at 260 nm, from the adenine ring. Only NADH absorbs at 340 nm. A clean NAD⁺ sample therefore shows essentially no absorbance at 340 nm, a quick check for contamination with the reduced form.

What should the certificate show? Identity by mass or HPLC against a reference, a purity figure, and ideally a note on water content, since the powder takes up moisture.

Specifications

• 1,000 mg per vial, supplied dry
• CAS 53-84-9
• Third-party analysis for identity and purity where a report has been commissioned; the reports we hold are on the certificates of analysis page
• Dispatched from within Canada

Purity and quality assurance

ExoLabz products undergo independent third-party testing to verify identity and purity, and results are published on this site. Researchers may run their own analytical verification. If validated testing confirms a purity discrepancy, ExoLabz provides a refund and testing reimbursement in line with company policy.

For research purposes only

NAD+ 1000mg is supplied strictly for laboratory research and analytical use. It is not intended for human consumption, veterinary use, clinical research involving human subjects, therapeutic use or diagnostic applications, and it has not been evaluated or approved by Health Canada for any such use. By purchasing, the buyer confirms the material will be handled only by qualified professionals and used solely for lawful laboratory research.

Compound identity and specification

Identity data for NAD+, published so the material can be checked against an independent record rather than taken on trust. The formula, mass and registry number below are verified against PubChem and can be looked up directly.

Analytical note — NAD+. This is a nucleotide coenzyme, not a peptide, and peptide methods do not apply to it. It absorbs strongly at 260 nm rather than at the peptide-bond wavelength, and the relevant purity question is the ratio of oxidised NAD+ to reduced NADH and to hydrolysis products, which a peptide-style HPLC report will not address.

Identity references The molecular formula, molecular mass and CAS registry number above are taken from the PubChem record linked in the table, not from a supplier datasheet, so every figure can be checked against an independent source in one click. Sequences are given as reported in the peptide literature, in the one-letter or three-letter convention as appropriate, with any non-standard residue named in full. Where a compound exists in more than one form — a free peptide and a metal complex, a fragment and its parent protein, a modified and an unmodified analog — the form supplied is the one identified here, and the mass on the certificate of analysis is what distinguishes it.

Research Literature

Peer-reviewed studies involving this compound and its class. All findings listed are from in vitro, cell culture or animal models and are provided for scientific reference only. This product is supplied for laboratory research and is not approved for human consumption, clinical, or veterinary use.

• Canto C, et al. Cell Metab. 2012;15(6):838-847. In mammalian cells and mouse skeletal muscle and brown adipose tissue, measured tissue NAD+ levels and SIRT1/SIRT3 target deacetylation. Source · PMID 22682224
• Gomes AP, et al. Cell. 2013;155(7):1624-1638. In aged mouse skeletal muscle, measured nuclear NAD+ decline, normoxic HIF-1alpha accumulation and loss of mtDNA-encoded OXPHOS subunits. Source · PMID 24360282
• Mills KF, et al. Cell Metab. 2016;24(6):795-806. In C57BL/6N mice over 12 months, measured tissue NAD+ synthesis, cellular energetics, insulin sensitivity and skeletal-muscle mitochondrial oxidative capacity. Source · PMID 28068222

Literature notes: These studies concern NAD+ precursors (nicotinamide riboside and nicotinamide mononucleotide) rather than NAD+ administered directly.

No third-party report has been published for this product yet. Every certificate we hold, with its laboratory verification key, is collected on the certificates of analysis page.

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