NAD+ 500mg

Description

NAD+ 500mg – Nicotinamide Adenine Dinucleotide, Research Grade

NAD⁺ (oxidised nicotinamide adenine dinucleotide, C₂₁H₂₇N₇O₁₄P₂, 663.4 g/mol) is the coenzyme that carries electrons for cellular dehydrogenases and is consumed as a substrate by sirtuins, PARPs and CD38. It is a dinucleotide, not a peptide. This 500 mg vial is the standard size for assay development and routine bench work. Supplied at 99%+ purity.

NAD+ 500mg is supplied as a dry powder for in vitro and laboratory research. It is not approved for human or veterinary use.

Measuring it: the methods laboratories actually use

Most questions about NAD⁺ in a model come down to measuring it accurately, and each method has a known weakness:

• UV absorbance. NAD⁺ and NADH both absorb at 260 nm, from the adenine ring; only NADH absorbs at 340 nm, with a molar absorptivity of about 6,220 M^-1cm^-1. Following the 340 nm signal is the classic way to track a dehydrogenase reaction. The weakness: in cell extracts, many other molecules also absorb at 260 nm.
• Enzymatic cycling assays. A pair of enzymes cycles the coenzyme between its oxidised and reduced forms while driving a coloured or fluorescent reporter, which amplifies very small amounts. They are sensitive, but measure NAD⁺ and NADH together unless the sample is split and one form is destroyed first.
• HPLC and LC-MS. Reversed-phase ion-pairing or HILIC chromatography separates NAD⁺ from NADH, ADP-ribose, nicotinamide and related metabolites, and mass spectrometry confirms each peak. This is the most specific approach, and it is the one used to check the identity and purity of the material itself.

Making solutions that stay accurate

The reason extraction and assay protocols look fussy is the chemistry. Oxidised NAD⁺ breaks down in alkaline solution and is comparatively stable in mild acid, while NADH is the reverse. Acid extraction is therefore used to preserve NAD⁺ and destroy NADH, and alkaline extraction does the opposite. That is how one sample can be split to measure each form separately.

For a working stock of NAD⁺:

• Dissolve in water or a neutral to slightly acidic buffer, not an alkaline one.
• Confirm the concentration by absorbance at 260 nm rather than relying on weight alone, because the powder takes up moisture from the air.
• Keep solutions on ice during use, prepare them fresh, and freeze any remainder in single-use aliquots.
• Let the sealed vial warm to room temperature before opening so moisture does not condense on the powder.

Questions researchers ask

Should I choose the 500 mg or the 1,000 mg vial? The 500 mg vial suits assay development and smaller series. The 1,000 mg vial suits high-throughput work where one batch should cover every condition.

Why is NAD+ listed with peptides? Laboratories studying mitochondrial and metabolic pathways often use both, for example alongside mitochondria-targeted peptides such as SS-31 or MOTS-c. Chemically it is a nucleotide, not a peptide.

What does the certificate show? Identity and purity by third-party analysis, published on this page.

Specifications

• 500 mg per vial, supplied dry
• CAS 53-84-9
• Third-party analysis for identity and purity, with the report published on this page
• Dispatched from within Canada

Purity and quality assurance

ExoLabz products undergo independent third-party testing to verify identity and purity, and results are published on this site. Researchers may run their own analytical verification. If validated testing confirms a purity discrepancy, ExoLabz provides a refund and testing reimbursement in line with company policy.

For research purposes only

NAD+ 500mg is supplied strictly for laboratory research and analytical use. It is not intended for human consumption, veterinary use, clinical research involving human subjects, therapeutic use or diagnostic applications, and it has not been evaluated or approved by Health Canada for any such use. By purchasing, the buyer confirms the material will be handled only by qualified professionals and used solely for lawful laboratory research.

Compound identity and specification

Identity data for NAD+, published so the material can be checked against an independent record rather than taken on trust. The formula, mass and registry number below are verified against PubChem and can be looked up directly.

Analytical note — NAD+. This is a nucleotide coenzyme, not a peptide, and peptide methods do not apply to it. It absorbs strongly at 260 nm rather than at the peptide-bond wavelength, and the relevant purity question is the ratio of oxidised NAD+ to reduced NADH and to hydrolysis products, which a peptide-style HPLC report will not address.

Identity references The molecular formula, molecular mass and CAS registry number above are taken from the PubChem record linked in the table, not from a supplier datasheet, so every figure can be checked against an independent source in one click. Sequences are given as reported in the peptide literature, in the one-letter or three-letter convention as appropriate, with any non-standard residue named in full. Where a compound exists in more than one form — a free peptide and a metal complex, a fragment and its parent protein, a modified and an unmodified analog — the form supplied is the one identified here, and the mass on the certificate of analysis is what distinguishes it.

Research Literature

Peer-reviewed studies involving this compound and its class. All findings listed are from in vitro, cell culture or animal models and are provided for scientific reference only. This product is supplied for laboratory research and is not approved for human consumption, clinical, or veterinary use.

• Canto C, et al. Cell Metab. 2012;15(6):838-847. In mammalian cells and mouse skeletal muscle and brown adipose tissue, measured tissue NAD+ levels and SIRT1/SIRT3 target deacetylation. Source · PMID 22682224
• Gomes AP, et al. Cell. 2013;155(7):1624-1638. In aged mouse skeletal muscle, measured nuclear NAD+ decline, normoxic HIF-1alpha accumulation and loss of mtDNA-encoded OXPHOS subunits. Source · PMID 24360282
• Mills KF, et al. Cell Metab. 2016;24(6):795-806. In C57BL/6N mice over 12 months, measured tissue NAD+ synthesis, cellular energetics, insulin sensitivity and skeletal-muscle mitochondrial oxidative capacity. Source · PMID 28068222

Literature notes: These studies concern NAD+ precursors (nicotinamide riboside and nicotinamide mononucleotide) rather than NAD+ administered directly.

The third-party report for this material is published on this page, and every certificate we hold is collected on the certificates of analysis page.

Related Research Compounds

Browse more compounds in Mitochondrial & Cellular Compounds, or view the full research peptide catalogue.

• NAD+ 1000mg
• SS-31 10mg
• MOTS-C 20mg
• MOTS-C 10mg