Racemisation and Chiral Purity in Synthetic Peptides
Every amino acid in a synthetic peptide is supposed to be the L form. Some fraction will not be…
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How research peptide identity and purity are verified, and how to read the supporting analytical documentation.
Every amino acid in a synthetic peptide is supposed to be the L form. Some fraction will not be…
A chromatogram shows peaks, and the arithmetic behind a purity figure assumes each peak is one compound. That assumption fails more often than the figure suggests.
A purity figure is the output of an instrument, and an instrument can be wrong. System suitability is the set of checks a laboratory runs before your sample is injected…
Deamidation is the most common chemical degradation route in peptides, and on a mass spectrum it announces itself as a shift of roughly 0.98 daltons.
A purity figure says how much of a sample is the target peptide. It says nothing about what the rest is, and the rest has identifiable origins.
A synthetic peptide is almost never supplied as a free base. It arrives as a salt, and which salt it is affects both your concentration calculations and, in some assay systems…
Weighing a lyophilised peptide accurately is harder than it looks, and the errors are systematic rather than random — they push in one direction and quietly bias every…
Two laboratories can analyse the same peptide and report different purity figures without either being wrong.
A purity percentage is a summary. The chromatogram it came from carries information the number discards, and reading it is a skill worth having if you buy peptides regularly.
Freeze-drying removes most of the water from a peptide preparation. It does not remove all of it…
"Research grade" is a commercial description, not a defined standard. Knowing what it does not include is more useful than knowing what it does.
"Third-party tested" appears on most peptide supplier sites. It is a meaningful claim only if you know who tested what, and when.
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